Title: Design and Analysis of Diagnostic Service Centers Abstract: Nurse lines are call centers that provide advice to patients about what the most appropriate course of action is according to their symptoms. For example, visiting the Emergency Department (ED) versus visiting a physician. At nurse lines, as well as many other service centers, diagnostic accuracy is a key performance measure in addition to waiting time due to congestion. Furthermore, since longer service typically entails higher accuracy but also more congestion, managers must decide on the optimal service depth and the staffing level to effectively perform a diagnostic process between an agent and a customer. We study this type of "diagnostic service center" in a strategic queueing setting, in which customers have autonomy to decide whether to use the service center or not, based on their expectation of diagnostic accuracy and waiting time. We model this problem as a multiple-server queueing system with the servers performing a sequential testing process. Analysis of our model shows that the optimal staffing level is such that all potential patients are enticed to use the nurse-line. Also, we show that when the optimal staffing level increases due to a change in the parameters of the environment, it is not necessarily accompanied with a decrease of the wait times. Finally, the intuition obtained from the analysis of our model allows improving rules of thumb in practical staffing decisions. Key words : service operations, strategic queueing, diagnostic process, call center Joint work with: Xiaofang Wang, Renmin University of China, Beijing, P.R.China 100872, xiaofang.wang@gmail.com; Laurens G. Debo, Graduate School of Business, University of Chicago, Chicago, IL 60637, ldebo@chicagogsb.edu; Stephen F. Smith, The Robotics Institute, Carnegie Mellon University, Pittsburgh, PA 15213, sfs@cs.cmu.edu